Journal: Bio-protocol

Article Title: Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons

doi: 10.21769/BioProtoc.3188

Figure Lengend Snippet:

Article Snippet: Neural Induction Medium 1 DMEM/F12 Medium 47.5 ml N2 supplement 0.5 ml B27 supplement 1 ml BSA 1 mg/ml NEAA 0.5 ml SB431542 10 μM Noggin 200 ng/ml Laminin 1 μg/ml Total: 50 ml Open in a separate window Neural Induction Medium 2 DMEM/F12 Medium 47.5 ml N2 supplement (N2-A from Stem Cell Technologies) 0.5 ml B27 supplement (SN-1 from Stem Cell Technologies) 1 ml BSA 1 mg/ml NEAA 0.5 ml Laminin 1 μg/ml Total: 50 ml Open in a separate window Note: Complete Neural Induction Medium 1 and 2 can be stored at 2-8 °C in the dark for up to 2 weeks.

Techniques:

Journal: Bio-protocol

Article Title: Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons

doi: 10.21769/BioProtoc.3188

Figure Lengend Snippet:

Article Snippet: Neural Induction Medium 1 DMEM/F12 Medium 47.5 ml N2 supplement 0.5 ml B27 supplement 1 ml BSA 1 mg/ml NEAA 0.5 ml SB431542 10 μM Noggin 200 ng/ml Laminin 1 μg/ml Total: 50 ml Open in a separate window Neural Induction Medium 2 DMEM/F12 Medium 47.5 ml N2 supplement (N2-A from Stem Cell Technologies) 0.5 ml B27 supplement (SN-1 from Stem Cell Technologies) 1 ml BSA 1 mg/ml NEAA 0.5 ml Laminin 1 μg/ml Total: 50 ml Open in a separate window Note: Complete Neural Induction Medium 1 and 2 can be stored at 2-8 °C in the dark for up to 2 weeks.

Techniques:

Journal: Bio-protocol

Article Title: Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons

doi: 10.21769/BioProtoc.3188

Figure Lengend Snippet:

Article Snippet: Neural Induction Medium 1 DMEM/F12 Medium 47.5 ml N2 supplement 0.5 ml B27 supplement 1 ml BSA 1 mg/ml NEAA 0.5 ml SB431542 10 μM Noggin 200 ng/ml Laminin 1 μg/ml Total: 50 ml Open in a separate window Neural Induction Medium 2 DMEM/F12 Medium 47.5 ml N2 supplement (N2-A from Stem Cell Technologies) 0.5 ml B27 supplement (SN-1 from Stem Cell Technologies) 1 ml BSA 1 mg/ml NEAA 0.5 ml Laminin 1 μg/ml Total: 50 ml Open in a separate window Note: Complete Neural Induction Medium 1 and 2 can be stored at 2-8 °C in the dark for up to 2 weeks.

Techniques:

Journal: Bio-protocol

Article Title: Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons

doi: 10.21769/BioProtoc.3188

Figure Lengend Snippet:

Article Snippet: Neural Induction Medium 1 DMEM/F12 Medium 47.5 ml N2 supplement 0.5 ml B27 supplement 1 ml BSA 1 mg/ml NEAA 0.5 ml SB431542 10 μM Noggin 200 ng/ml Laminin 1 μg/ml Total: 50 ml Open in a separate window Neural Induction Medium 2 DMEM/F12 Medium 47.5 ml N2 supplement (N2-A from Stem Cell Technologies) 0.5 ml B27 supplement (SN-1 from Stem Cell Technologies) 1 ml BSA 1 mg/ml NEAA 0.5 ml Laminin 1 μg/ml Total: 50 ml Open in a separate window Note: Complete Neural Induction Medium 1 and 2 can be stored at 2-8 °C in the dark for up to 2 weeks.

Techniques:

Journal: Cell reports

Article Title: Spatially non-overlapping Ca 2+ signals drive distinct forms of neurotransmission

doi: 10.1016/j.celrep.2023.113201

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: B-27 supplement , Promega , Catalog # 17504–010.

Techniques: Recombinant, Transfection, Generated, Plasmid Preparation, Software, Inverted Microscopy

Journal: Cell Journal (Yakhteh)

Article Title: The Effect of Biomaterials on Human Dental Pulp Stem Cell Neural Differentiation: A Scoping Review

doi: 10.22074/CELLJ.2023.2007711.1375

Figure Lengend Snippet: The effect of biomaterials on hDPSC neural differentiation

Article Snippet: (5) , Pisciotta et al. , 2018 , hDPSC , In vitro , 3D floating sphereculture system , EGF, bFGF, DMEM/F12 culture medium,L-glutamine penicillin,streptomycin, B27 , Indirect , IF analysis, Western blot, pseudocolour analysis , Nestin,CD271, SOX-10, β-III tubulin, MAP2 , Neural progenitor cells, neural crest stemcells.

Techniques: In Vitro, Cytometry, Marker, Western Blot, Staining, In Vivo, Immunohistochemical staining, Real-time Polymerase Chain Reaction, Immunostaining, Flow Cytometry, Suspension, Modification, Enzyme-linked Immunosorbent Assay